Wednesday, 7 October 2015

PoliticalSpeak: brain health initiative finally launched

Time is brain; pledge your support. #BrainHealth #PoliticalSpeak #MSBlog #MSResearch #ECTRIMS2015

"Despite being in Barcelona 3 days today is day 1 of ECTRIMS 2015. After a year's work we finally managed to launch our "Brain Health: time matters in multiple sclerosis" policy document last night. We would have preferred an official ECTRIMS satellite symposium, which was, unfortunately, not to be. Despite this we had a very good turnout and there has been a lot of discussion around the document. What we now need is your support and help in disseminating the information. Please download the document, read it and if you agree with it pledge your support for the document. We are hoping the campaign goes viral and we get thousands of people signing up to the initiative. I truly believe that if our recommendations are adopted and implemented widely it will make a big difference to people living with MS and their families."

"To pledge your support clinic on this image below and sign-up."

"We need your help to make this campaign go viral. At present we are working on the concept of holding a competition to see who can come up with the greatest viral campaign. The latter could be a picture, poem, movie, short story, sculpture, drawing, animation, design piece, campaign, etc. What will it take for the MS community to take Brain Health seriously? The following short video is an example of a very effective app that was designed by a company called periscopic to show you the impact of gun crime in USA; to keep it short I have only captured a stats from a single year (2013). The app shows you how many citizens in the USA died prematurely as a result of gun crime and how many years of life were lost. I would love someone to make an app like this for MS to illustrate how many neurons/axons, or brains, or cognitive functions are lost in MSers waiting to be diagnosed, waiting to be treated, waiting for a monitoring MRI, or waiting for their therapy to be escalated to a more effective treatment when they are not responding to their current therapy.  In essence we want to communicate the concept that 'time is brain'."

CoI: multiple

Time is Brain-Policy Document 


ProfG will post on this, when he has time in between his ECTRIMS engagements.

What do you think....A no brainer? 
Apparently not for Some Neuros!!!!!!! 

                                          Time to Change

ECTRIMS 2015 Social Media

If you are Neuro and your are at ECTRIMS 2015 Today

Come along and see why you need to be Social Media Savvy

See you there on Wednesday lunchtime

Social Media Teaching Course:
  • Wednesday 7th Oct
  • 12.30-14.00

Autoimmunity in SPMS, Its all over the place.

Sjöberg R, Mattsson C, Andersson E, Hellström C, Uhlen M, Schwenk JM, Ayoglu B, Nilsson P.Exploration of high-density protein microarrays for antibody validation and autoimmunity profiling. N Biotechnol. 2015. pii: S1871-6784(15)00154-5. doi: 10.1016/j.nbt.2015.09.002. [Epub ahead of print]

High-density protein microarrays of recombinant human protein fragments, representing 12,412 unique Ensembl Gene IDs, have here been produced and explored. These protein microarrays were used to analyse antibody off-target interactions, as well as for profiling the human autoantibody repertoire in plasma against the antigens represented by the protein fragments. Affinity-purified polyclonal antibodies produced within the Human Protein Atlas (HPA) were analysed on microarrays of three different sizes, ranging from 384 antigens to 21,120 antigens, for evaluation of the antibody validation criteria in the HPA. Plasma samples from secondary progressive multiple sclerosis patients were also screened in order to explore the feasibility of these arrays for broad-scale profiling of autoantibody reactivity. Furthermore, analysis on these near proteome-wide microarrays was complemented with analysis on HuProt™ Human Proteome protein microarrays. The HPA recombinant protein microarray with 21,120 antigens and the HuProt™ Human Proteome protein microarray are currently the largest protein microarray platforms available to date. The results on these arrays show that the Human Protein Atlas antibodies have few off-target interactions if the antibody validation criteria are kept stringent and demonstrate that the HPA-produced high-density recombinant protein fragment microarrays allow for a high-throughput analysis of plasma for identification of possible autoantibody targets in the context of various autoimmune conditions.

In this study a chip with lots of proteins fragments on it (up to 21,000) was made and blood from pwSPMS were used to probe it. When they tested rabbit antibodies they largely targetted a dominant protein but some were rubbishand we know this from buying lots of antibodies and they don't do what they say on the tin and have multiple targets. So what happened in SPMS. There were lots of autoreactivity meaning that antibodies reacting to targets were present, but there was no overlap in what those targets were and there were differences if the target was a whole protein of a fragment of a protein. However myelin basic protein is not jumping out. The problem is cause and effect also. So is the antibody causing the damage or as a consequence of damage, you get release of proteins and they can stimulate an antibody response.